TOPIC; NANO DRUG DELIVERY SYSTEM IMPROVES ISOPRENALINE-INDUCED CARDIOMYOCYTE INJURY
Content of the open report: (including issues to be studied or solved, research methods used, and literature review, not less than 2,000 words
The problem to be solved:
With the aging of the population, changes in dietary structure and increased work pressure, cardiovascular diseases have become major diseases that seriously endanger human health and quality of life. Including myocardial infarction (MI) and heart failure, even with the most advanced pharmacological and medical device treatments, the mortality and morbidity of heart disease remains high. Cardiomyocytes are terminally differentiated cells that do not have the ability to divide and regenerate. When myocardial infarction occurs, a large number of myocardial cells die, causing irreversible damage to the heart tissue and a significant decrease in cardiac function. And the clinically used myocardial infarction treatment drugs, such as aspirin, atorvastatin, captopril, etc., due to the lack of targeting of the myocardial infarction, a large amount of drugs and a long time of use, can easily cause the body to accumulate toxic side effect. Therefore, the purpose of this topic is to explore new therapeutic methods to protect cardiomyocytes and improve cardiac function.
Adopted research methods:
(1) Synthesis of Nano Drug Delivery System. In view of the lack of targeting defects in cardiac lesions for myocardial infarction drugs, research and design of nanomaterials micelles with synthetic silicon core as the core, due to increased vascular epithelial permeability at the myocardial infarction site, silicone bundles can be passed through the intravenous injection into the blood Leakage is passively targeted to the site, thereby improving the function of the damaged myocardium.
(2) Establishment of myocardial cell injury model. In experiments, isoproterenol (ISO) was used to establish a myocardial injury model. Different concentrations of NE were co-incubated with cardiomyocytes and the dose-effect relationship was analyzed. Select the optimal concentration and time to establish the damage model.
(3) Analysis of indicators of myocardial damage. MTT assay and LDH leakage assay were used to detect the extent of cardiomyocyte damage; oxidative stress level was detected using the active oxygen ROS kit; morphological changes were observed using HE and Giemsa staining
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